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Features at a glance
Brookhaven's’ NanoBrook 173Plus instrument is the go-to-solution for characterization of proteins, antibodies, nanoparticles, macromolecular assemblies and other molecules/particles of small sizes. The NanoBrook 173Plus uses backscattering detection for the highest sensitivity and accuracy optimized for molecules and particles less than a few tens of nanometers. Detection at 90° is used for larger particles.
Reliable, Accurate, and Accurate Analysis
The new NanoBrook 173Plus particle size analyzer incorporates all you need for fast, routine, sub-micron measurements. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, most measurements only take a minute or two.
Principles of Operation - Nanoparticles
Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents, if required. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. At 173° sample volume may be reduced to 50 µL with a polystyrene, U-shaped, disposable cuvette and the sample is recoverable. At 90° square polystyrene or glass cells (two or three mL) are used, one as small as 10 µL (non-disposable). In addition, disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. In all case, just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 173Plus.
Speed and low concentration/small size capabilities
The NanoBrook 173Plus utilizes backscattering to measure measures using backscattering the size of the proteins/particles by building the correlation function. The smoother the function, the better the results. For small molecules, the scattering intensity is typically low and the measurement takes longer to get enough statistics from the photons counted to obtain a good correlation function.The backscattering angle makes for a larger scattering volume, measuring more proteins/particles for a quick measurement of their sizes. The advantage of the backscattering is clear for size below 10 nm.
The NanoBrook 173Plus particle size analyzer offers many choices for data analysis. One choice is the lognormal distribution by: intensity, volume, surface and number. Another choice, the full size distribution (MSD) can be displayed by the same four weighting factors. In the example above, the MSD is presented by volume for lysozyme. The size distribution and cumulative distribution are shown in the table on the right. Lysozyme has a very low polydispersity in this case.
Sample Type Most globular proteins, nanoparticles, and small polymers in any non-absorbing liquid Size Range > 0.3 nm to 10 µm diameter, depending on refractive index and concentration Sample Cells 50 µL disposable, 1 to 3 mL disposable plastic, 40 µL quartz flow cell, 10 µL quartz minimum Concentration Range 0.1 ppm to 50 mg/mL, depending on refractive index and concentration Signal Processing Dynamic Light Scattering, DLS Correlator Brookhaven's TurboCorr, multitau research grade with 510 hardware channels, covering the equivalent of 1010 linearly-spaced channels, 100% efficiency, real-time operation over the entire delay-time range Precision ± 1% typically Temperature Control -5 °C to 110 °C, ± 0.1 °C, active control. No external circulator required. Condensation Control Purge facility using dry air, nitrogen preferred Standard Laser 40 mW red diode laser, nominal 640 nm wavelength Scattering Angle 173° & 90° Data Presentation Average & width, lognormal fit, and multimodal size distribution standard Compliance ISO 13321 and ISO 22412 compliant results Power Requirements 100/115/220/240 VAC, 50/60 Hz, 150 Watts Dimensions 23.3 x 42.7 x 48.1 (HWD) Weight 15 kg Environmental Characterisitics
Temperature 10 °C to 75 °C
Humidity 0% to 95%, non-condensing
CE Certificate Class I laser product, EN 60825-1:2001 CDRH
90PDP Absolute molecular weights of polymers in solution. Option includes 40 µL flow cell (90PFC) and Debye plot software. Relative molecular weight calculated from the DLS determination of the diffusion coefficient is standard. BI-PSD21 21 CFR Part 11 software to assist ERES compliance. BI-SFS Sample filtration system provides fast and convenient way to clean your light scattering samples, a necessity for samples smaller than 20nm. Utilizing Teflon® tubing and nonreactive components, the BI-SFS is compatible with most sample filtration needs provided there is at least 5 mL of liquid. For protein and other rare samples, use 20 nm BI-DF20AQ Anatop filters instead. Lasers Additional options available; contact us for more information.
Cells BI-SCP Box of 100 plastic cells with caps, 2.2 to 4.5 mL volume. Use with water, for sizing and zeta. BI-SM50 Box of 80 plastic cells, disposable, 50 µL volume, sample retrievable, requires BI-SM50A. Use with water. BI-SM50A One sample cell adaptor for use with BI-SM50. BI-SM10 One 10 µL cell for sizing of small and rare samples, sample is retrievable. 90PFC Additional 40 µL quartz flow cell for use with 90PDP Reference Materials BI-LTX92 Latex reference material for validation for sizing, 15 mL, 92 ± 3 nm. BI-SVK92 Starter validation kit for sizing, 2 mL, 92 ± 3 nm. BI-CC Nitrogen-purge facility for condenstation control.v
Note: Current users of the 173Plus may upgrade to include zeta potential provided their instrument has the necessary mechanics, optics and hardware. Contact the factory for more information.